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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-9496 is a subtype B CXCR4 virus that is based on Human Immunodeficiency Virus-1 NL4-3 gp41 D36G (ARP-9489) with substitutions relative to the subtype B consensus sequence in gp41 amino acids 36-45 which confer decreased susceptibility to Enfuvirtide (T-20). The noted mutations were introduced into pNL4-3 and the DNA was transfected into 174xCEM cells. Virus was collected after maximum cytopathic effects were noted. ARP-9496 is part of a panel of NL4-3 viruses that were constructed to study resistance to the fusion inhibitor Enfuvirtide.
Virus stocks are typically grown in CEM CD4+ cells or MT-4 cells. Otherwise, the virus should be able to be produced in any other receptive target cell. Virus is cytopathic and will produce large syncytia.
ARP-9496 is negative for mycoplasma, bacteria and fungi.
Each vial contains approximately 1 mL of cell-free virus.
Please refer to the attached file(s) for additional information.
If shipping to the U.S. State of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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