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-70°C or colder
ARP-4690 is a universal lentiviral self-inactivated
transducing vector that contains a chimeric 5' CMV-TATA enhancer/promoter site
and a 3' bovine growth hormone polyA signal. The backbone is derived from pUC18
(of pNL4-3). This HIV-1 derived transducing vector contains the following HIV
sequences: RU5, UTR without the major splice site, 720 base pair gag, 927 base pair env including the RRE, the two integration att sites and the 3'R.
NR-4690 was generated by combining the lentiviral
transducing vector pTV, self-inactivating lentiviral vector
pTYΔCT-CMVnlacZdl3'U3#1U5pA (see figures in plasmid and sequence file) and 5'
splice site and env deletion env.dl.6 (see figures in plasmid and sequence file)
with a NotI-EcoRI linker. Unique cloning sites on the linker that can be used
for foreign gene insertion are: NotI,
NheI, SalI, SmaI, BamHI, SacII, SpeI, EcoRI and KpnI.
Plasmids can be propagated in Escherichia coli (E. coli)
STBL2 cells and grown at 37°C. Larger plasmids may benefit from growth at 30°C.
This construct may also be grown in other competent cells. For additional
details refer to the provided protocol for DNA transfection for production of
recombinant lentiviruses, and the plasmid map and sequence files.
Each vial of ARP-4690 contains
approximately 5 µg of dried purified DNA stabilized in DNAstable®PLUS. Please refer to the appropriate
data sheet for lot-specific information.
Chang, L.-J., et al. “Efficacy and Safety Analyses of a
Recombinant Human Immunodeficiency Virus Type 1 Derived Vector System.” Gene Ther. 6 (1999): 715-728.
Iwakuma, T., Y. Cui Y and L.-J. Chang. “Self-Inactivating
Lentiviral Vectors with U3 and U5 Mutations.” Virology 261
(1999): 120-132. PubMed:15750613.
Cui, Y., T. Iwakuma and L.-J.
Chang. “Contributions of Viral Splice Sites and Cis-Regulatory Elements to
Lentivirus Vector Function.” J.
Virol 73 (1999): 6171-6176.PubMed: 10364378.
Scientists at for-profit institutions or who intend
commercial use of this reagent must contact UF Innovate at the following email
before the reagent can be released.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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