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-70°C or colder
is a low copy number, 5215 base pair plasmid useful in stabilizing lentivirus
sequences such as SIV, EIAV, Visna maedi, etc. for subcloning, propagation, and
manipulation in Escherichia coli (E.
coli). This plasmid reduces the level of toxic gene expression from cryptic
promoter sequences. The sequence is available from GenBank: AF035682. pLG338/30
contains the 3.05 kb PvuII-HincII fragment from pLG338, which, in
turn, contains the pSC101 ori. A 1.65 kb EcoRI-HaeII fragment that includes the pBR322
ampicillin resistance gene, and the multiple cloning site (FspI-EcoRI)
from pIBI30 are also present in this plasmid. For additional details please refer to the
provided plasmid map. The ampicillin-resistant E. coli, DH5a (glycerol
stock) should be cultured at 37°C
vial of ARP-3923 contains ampicillin resistant E. coli, DH5a transformed with pLG338/30. Please refer to
the appropriate data sheet for lot-specific information.
Cunningham, T. P., R. C. Montelaro and K. E. Rushlow. “Lentivirus
Envelope Sequences and Proviral Genes are Stabilized in Escherichia coli
when Cloned in Low Copy Number Plasmid Vectors.” Gene124
(1993): 93-98. PubMed: 8382658.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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