Disclaimer: You are now leaving www.HIVReagentProgram.org and are going to a website that is not operated by NIH HIV Reagent Program. We are not responsible for the content or availability of linked sites.
ABOUT THIRD PARTY LINKS ON OUR SITE:
NIH HIV Reagent Program offers links to other third party websites that may be of interest to our website visitors. The links provided in our website are provided solely for your convenience and may assist you in locating other useful information on the Internet. When you click on these links, you will leave the NIH HIV Reagent Program website and will be redirected to another site. These sites are not under the control of NIH HIV Reagent Program.
NIH HIV Reagent Program is not responsible for the content of linked third party websites. We are neither an agent for these third parties nor do we endorse or guarantee their products. We make no representation or warranty regarding the accuracy of the information contained in the linked sites. We suggest that you always verify the information obtained from linked websites before acting upon this information.
Please read third party privacy and security policies closely as these may be different than NIH HIV Reagent Program policies.
If you have any questions or concerns about the products and services offered on linked third party websites, please contact the third party directly.
-70°C or colder
Quantity limit per order
for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-1525 [HIV-1 LTR CAT reporter vector (pCD54)] is a deletion
mutant where the nef region is deleted from the HIV LTR and the NFκB and Sp-1
nuclear factor binding sites are deleted at -48. To produce this vector clone pC15CAT (BEI
Resources ARP-1527)a derivative of pSV0CAT, was cleaved with KpnI, treated with Bal31 exonuclease,
blunted and the XbaI linkers ligated. The resultant deletion mutant, pCD54,
contains HIV-1IIIB LTR sequences from -48 to +80 located in front of the CAT
bacterial host is Escherichia coli,
strain DH5α but strain HB101 is also an acceptable host.
vial of ARP-1525 contains 1 mL of ampicillin resistant E. coli, strain DH5α
transformed with pCD54. Please refer
to the appropriate data sheet for lot-specific information.
Gorman, C. M., L. F. Moffat and B. H. Howard. “Recombinant
Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells.” Mol. Cell Biol. 2 (1982):1044-1051.
Arya, S. K., et al. “Trans-Activator
Gene of Human T-Lymphotropic Virus Type III (HTLV-III).” Science229 (1985):69-73. PubMed:
Seikevitz, M., et al. “Activation
of the HIV-1 LTR by T Cell Mitogens and the Trans-Activator Protein of HTLV-I.”
Science 238 (1987):1575-1578.
K. S., w. T. Liu and S. F. Josephs. “Regulation of Cellular Trans-Activating
Activities in Two Different Promonocytic Leukemia Cell Lines.” Cancer Lett. 60 (1991):75-83.
L. J., et al. “Transactivation Induced by Human T-Lymphotropic Virus Type III
(HTLV III) Maps to A Viral Sequence Encoding 58 Amino Acids and Lacks Tissue
Specificity.” Virology 148 (1986): 226-231. PubMed:
for-profit institutions or who intend commercial use of Release Category C
Reagents (Cat# ARP-1525) must contact Dr. Brian W. Bailey, NIAID Office of
Technology Development, 6610 Rockledge Drive, Room 4036, MSC 6606, Bethesda, MD
20892-6606, Email: email@example.com, Tel: 301-594-1697, Fax: 301-402-7123,
before the reagent can be released.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
Knowledge Base (FAQs)
Microsoft PowerPoint Viewer
Microsoft Word Viewer
Microsoft Excel Viewer
Call Us Toll-Free:+1 (888) 487-0727
TTY:(703) 365 - 2727 (for deaf, hard of hearing, or speech impaired)