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-70°C or colder
Quantity limit per order
for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-1523 [HIV-1 LTR CAT reporter vector (pCD16)] is a deletion
mutant where the 5' end of the HIV LTR to
position -176 from the transcriptional start site is deleted. To produce this
vector clone pC15CAT (BEI Resources ARP-1527) a derivative of pSV0CAT, was cleaved with KpnI, treated with Bal31 exonuclease,
blunted and the XbaI linkers ligated. The resultant deletion mutant, pCD16,
contains HIV-1IIIB LTR sequences from -176 to +80 located in front of the CAT
bacterial host is Escherichia coli,
strain DH5a but strain HB101 is also an acceptable host.
vial of ARP-1523 contains 1 mL of ampicillin resistant E. coli, strain DH5a
transformed with pCD16. Please refer
to the appropriate data sheet for lot-specific information.
Gorman, C. M., L. F. Moffat and B. H. Howard. “Recombinant
Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells.” Mol. Cell Biol. 2 (1982):1044-1051.
Arya, S. K., et al. “Trans-Activator
Gene of Human T-Lymphotropic Virus Type III (HTLV-III).” Science229 (1985):69-73. PubMed:
Seikevitz, M., et al. “Activation
of the HIV-1 LTR by T Cell Mitogens and the Trans-Activator Protein of HTLV-I.”
Science 238 (1987):1575-1578.
K. S., w. T. Liu and S. F. Josephs. “Regulation of Cellular Trans-Activating
Activities in Two Different Promonocytic Leukemia Cell Lines.” Cancer Lett. 60 (1991):75-83.
L. J., et al. “Transactivation Induced by Human T-Lymphotropic Virus Type III
(HTLV III) Maps To A Viral Sequence Encoding 58 Amino Acids and Lacks Tissue
Specificity.” Virology 148 (1986): 226-231. PubMed:
for-profit institutions or who intend commercial use of Release Category C
Reagents (Cat# ARP-1523) must contact Dr. Brian W. Bailey, NIAID Office of
Technology Development, 6610 Rockledge Drive, Room 4036, MSC 6606, Bethesda, MD
20892-6606, Email: firstname.lastname@example.org, Tel: 301-594-1697, Fax: 301-402-7123,
before the reagent can be released.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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