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Quantity limit per order
for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-1520 [HIV-1 LTR CAT reporter vector (pCD54E8)] is a deletion
mutant where the 5' end of the LTR to
position -48 from the transcriptional start site is deleted, however, the
presence of the NFκB enhancer sequence results in restored transcriptional
activity as determined by CAT assay. The LTR sequences are cloned upstream to
the CAT gene. To produce this vector clone pC15CAT (BEI Resources
derivative of pSV0CAT, was cleaved
with KpnI, treated with Bal31 exonuclease, blunted and the XbaI linkers ligated.
The resultant deletion mutant, pCD54E8, contains HIV-1IIIB LTR sequences from
-48 to +80 located in front of the CAT gene. The NFκB sequence has been cloned
into the XbaI site at -48 in the reversed orientation. The NFκB insert
sequence is: XbaI XbaI -48 5'-cgcagcgagtcact/ctagatggaaagtccccagcggaaagtccct/ctagag*gcgag-3'.
bacterial host is Escherichia coli,
strain DH5α but strain HB101 is also an acceptable host.
vial of ARP-1520 contains 1 mL of ampicillin resistant E. coli, strain DH5α transformed with pCD54E8. Please refer
to the appropriate data sheet for lot-specific information.
Gorman, C. M., L. F. Moffat and B. H. Howard. “Recombinant
Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells.” Mol. Cell Biol. 2 (1982):1044-1051.
Arya, S. K., et al. “Trans-Activator
Gene of Human T-Lymphotropic Virus Type III (HTLV-III).” Science229 (1985):69-73. PubMed:
Seikevitz, M., et al. “Activation
of the HIV-1 LTR by T Cell Mitogens and the Trans-Activator Protein of HTLV-I.”
Science 238 (1987):1575-1578.
K. S., w. T. Liu and S. F. Josephs. “Regulation of Cellular Trans-Activating
Activities in Two Different Promonocytic Leukemia Cell Lines.” Cancer Lett. 60 (1991):75-83.
L. J., et al. “Transactivation Induced by Human T-Lymphotropic Virus Type III
(HTLV III) Maps To A Viral Sequence Encoding 58 Amino Acids and Lacks Tissue
Specificity.” Virology 148 (1986): 226-231. PubMed:
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
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Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
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For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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