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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-588 is the fusion product of CEM-A and normal peripheral blood mononuclear cells. CEM-A cells express abundant cell surface CD4 and are well suited to human immunodeficiency virus (HIV) infection. HIV infection of CEM-A cells results in easily detectable multinucleated cells.
Growth Characteristics: Remove cells from monolayers with enzyme-free dissociation solution. CEM-A cells will reattach to plastic or glass in approximately 5 minutes. Once the culture is established, cells are maintained in T-75 flasks and subcultured every 4 to 5 days. The doubling time is 18 to 24 hours. Cells grow as even monolayers and do not form clumps. Cells must be maintained in hypoxanthine and thymidine (HT)-containing medium to be infectable by HIV.
Growth Medium: RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 1% hypoxanthine-thymidine (HT) solution
ARP-588 is negative for bacteria, Mycoplasma, yeast and mold.
Each vial of ARP-588 contains
approximately 3.8 × 106 cells in growth medium containing 7.5% dimethyl sulfoxide (DMSO). Please refer to the
appropriate data sheet for lot-specific information.
Tremblay, M., et
al. “New CD4(+) Cell Line Susceptible to Infection by HIV-1.” J. Med. Virol.
28 (1989): 243-249. PubMed: 2570823.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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