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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-462 is a culture of WE17/10 cells, a mature human T lymphocyte derived from a patient with a slow-growing T-cell lymphoma. ARP-462 is interleukin-2 (IL-2)-dependent and express surface CD2, TCR/CD3, CD4, CD25, CD29, CD45RO, CD71, Leu8, and HLA class I and class II antigens.
ARP-462 does not contain integrated proviral sequences from known human retroviruses. The karyotype is abnormal [47, XX, -3, +der(3), t(1;3)(q41;q29) +M] in 100% of the cells.
Growth Characteristics: ARP-462 grows in a single cell suspension at high densities and in clumps at low cell densities. It is recommended that the cells be used at passage numbers below 50 for initiating long term infection experiments, and below 120 for other studies. This prevents the cells from acquiring characteristics resulting from long term tissue culture.
Growth Medium: RPMI 1640 medium supplemented with 20% fetal bovine serum, 10 mM HEPES, 30 to 100 units per mL human IL-2, 1.25 mM L-glutamine, 0.55 mM L-arginine HCl and 0.24 mM L-asparagine
ARP-462 is negative for Mycoplasma, bacteria and fungi.
Each vial of ARP-462 contains
approximately 7 × 106 cells per mL in growth medium containing 10% dimethyl sulfoxide (DMSO). Please refer to the
appropriate data sheet for lot-specific information.
K. F. Van de Keere and R. Kettmann. “A Specific Defect in CD3-? Gene Transcription Results in Loss of T Cell Receptor/CD3 Expression Late
after Human Immunodeficiency Virus Infection of a CD4+ T Cell line.”
Proc. Natl. Acad. Sci. USA 87 (1990): 6713-6717. PubMed: 2144349.
K. “Two-dimensional gel analysis of gene expression in human cell lines
established from patients with T-cell malignancies.” Recent Progress in Two-dimensional Electrophoresi. (1986)
Galteau, M.M. and G. Siest (Eds.), Press. Univ. Nancy.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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