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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-3337 are HeLa-derived HtTa-1 cells transfected with HXBΔPΔEnv, T1RevEnv and HM272 (hygromycin resistance gene construct) and can be induced to express human immunodeficiency virus 1 (HIV-1) Gag, Pol, Rev and Env proteins. Clone HXBΔPΔEnv-T1RevEnv is a selected clone of this transfection.
HIV-1 Gag, Pol, Rev and Env proteins can be induced by removing tetracycline from the culture medium. Peak protein expression occurs 6 to 7 days after tetracycline removal. The cells have been continuously maintained in tetracycline medium. During passage, both PBS and trypsin should also contain 2.0 µg per mL tetracycline.
Growth Medium: DMEM supplemented with 10% fetal bovine serum, 0.2 mg per mL G418, 0.1 mg per mL hygromycin and 2.0 µg per mL tetracycline
ARP-3337 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-3337 contains
approximately 2 × 106 cells in DMEM supplemented with 20% fetal bovine serum, 2.0 µg per mL tetracycline and 10% dimethyl sulfoxide (DMSO). Please refer to the
appropriate data sheet for lot-specific information.
Yu, H., et al. “Inducible
Human Immunodeficiency Virus Type 1 Packaging Cell Lines.” J. Virol. 70
(1996): 4530-4537. PubMed: 8676479.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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