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-100°C or colder, preferably in the vapor phase
of a liquid nitrogen freezer
limit per order for this item is 1. This item can be ordered twice a year. Orders
over this limit will be sent to NIAID for approval before shipment.
ARP-166 is a hypoxanthine-aminopterin-thymidine (HAT)-sensitive derivative of CEM cells that support human
immunodeficiency virus 1 (HIV-1) replication. Strain CEM is a human T-cell line derived
from the peripheral blood buffy coat of a four-year-old Caucasian female with acute
Strain A3.01 was selected by growth in hypoxanthine and
aminopterin-containing medium. It is suitable for human T-lymphocyte fusions.
Cells are Leu-3+, Leu-8+, Leu-1+,tac-, transferrin receptor+, sensitive to infection with lymphadenopathy associated virus (LAV), and susceptible to cytopathic effects when infected.
Growth Characteristics: When thawing,
gently wash out the DMSO with 37°C medium and seed the initial culture at 1 × 106 cells per mL. Cells quickly recover viability over 3
days. Passage the cells every three days to give a concentration
of 1 × 106 cells per mL. Cells grow in single cell suspension with
a mature lymphocyte morphology and has a doubling time of 24 hours. A3.01 has
also been grown successfully in OPTI-MEM® Reduced
Sera Medium (Gibco™) containing 2.5% fetal bovine serum, 2.0 mM L-glutamine, 100 U per mL
penicillin and 100 µg per mL streptomycin.
Propagation Medium: RPMI 1640 medium supplemented
with 10% fetal bovine serum.
ARP-166 is negative forbacteria, Mycoplasma and fungi.
Each vial of ARP-166 contains
approximately 5 × 106 cells in RPMI 1640 medium
supplemented with 10% fetal bovine serum and 10% dimethyl
sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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