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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders
over this limit will be sent to NIAID for approval before shipment.
ARP-11440 is a full-length HIV-1 infectious molecular clone containing the V1-V3
envelope regions of Ba-L in a NL4-3 background. The combined size of vector and insert is approximately 14
kilobases and contains the gene for ampicillin resistance.
Note: Plasmids can be propagated in STBL2 cells and grown at
37°C. Larger plasmids may benefit from growth at 30°C. This construct may also
be grown in other competent cells.
Each vial of ARP-11440 contains approximately 5 µg of dried,
purified DNA stabilized in DNAstable®Plus (Biomatrica®). Please refer to the
appropriate data sheet for lot-specific information.
Chesebro, B., et al. “Identification of Human
Immunodeficiency Virus Envelope Gene Sequences Influencing Viral Entry into
CD4-Positive HeLa Cells, T-Leukemia Cells, and Macrophages.” J. Virol.
65 (1991): 5782-5789. PubMed: 1920616.
Chesebro, B., et al. “Macrophage-Tropic Human Immunodeficiency
Virus Isolates from Different Patients Exhibit Unusual V3 Envelope Sequence
Homogeneity in Comparison with T-Cell Tropic Isolates: Definition of Critical
Amino Acids Involved in Cell Tropism. J. Virol. 66 (1992):
6547-6554. PubMed: 1404602.
Toohey, K., et al. “Human Immunodeficiency Virus Envelope V1
and V2 Regions Influence Replication Efficiency in Macrophages by Affecting Virus
Spread. Virology 213 (1995): 70-79.
Brandon L., et al. “Role of Low CD4 Levels in the Influence
of Human Immunodeficiency Virus Type 1 Envelope V1 and V2 Regions on Entry and
Spread in Macrophages.” J. Virol. 79 (2005): 4828-4837. PubMed:
Scientists at for-profit institutions or who intend
commercial use of Release Category C Reagent must contact Dr. Brian W. Bailey,
NIAID Office of Technology Development, 6610 Rockledge Drive, Room 4036, MSC
6606, Bethesda MD 20892-6606, Tel: 301-594-1697, Fax: 301-402-7123, Email: mailto:firstname.lastname@example.org, and specify the name and a
description of the intended use of the reagent, before the reagent can be
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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